End‐to‐end image analysis pipeline for liquid‐phase electron microscopy

MiPipeline (Microscopy Pipeline): A User Friendly Software Environment for Microscopy Image Analysis and Informatics

Recent advances in the field of optical microscopy have enabled scientists to image complex biological processes across a wide range of spatial and temporal resolutions, resulting in an exponential increase in optical microscopy data. There is now a need for a computing environment that provides accurate, high-throughput image analysis, in conjunction with data provenance, easily accessible wor...

Isolation of Herpetosiphon giganteus and Ultrastructure Analysis by Electron Microscopy

Herpetosiphon giganteus is a filamentous gliding bacterium. Gliding motility is the movement of the cells over surfaces without the aid of flagella. The mechanism responsible for bacterial gliding motility has not been known and there are only a few data on Herpetosiphon giganteus. The aim of this study was to observe the ultrastructure and negative staining of isolated strains of Herpetosiphon...

Image Analysis and Interactive Visualization Techniques for Electron Microscopy Tomograms

Images are an important data source in modern science and engineering. A continued challenge is to perform measurements on and extract useful information from the image data, i.e., to perform image analysis. Additionally, the image analysis results need to be visualized for best comprehension and to enable correct assessments. In this thesis, research is presented about digital image analysis a...

Fast Noise-image Registration for Electron Microscopy

1. INTRODUCTION Single particle electron microscopy (EM) has become the most powerful technique in structural biology for studying large macromolecules and their assemblies. At near physiological conditions, the 3D structure of relevant biological complexes can be determined from sub-nanometer to even near atomic resolution (Frank, 2006). Using a small amount of purified sample, researchers pro...

Electron Microscopy of Fowl Pox Virus